14.9 Folate:Active B12

14.9.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 146.869451 1 <0.0001 ***
folate_Active.B12 3.973972 1 0.05 ns
Study 7.725711 1 0.005 **
GA_samp 80.489628 1 <0.0001 ***
folate_Active.B12:Study 6.300094 1 0.01 *
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 26.4615227 1 <0.0001 ***
folate_Active.B12 0.5606844 1 0.5 ns
Study 12.9049475 1 0.0003 ***
GA_samp 1318.5704897 1 <0.0001 ***
folate_Active.B12:Study 3.2107060 1 0.07 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 246.688649 1 <0.0001 ***
folate_Active.B12 1.758414 1 0.2 ns
Study 1.272412 1 0.3 ns
GA_samp 619.142889 1 <0.0001 ***
folate_Active.B12:Study 1.405998 1 0.2 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 1.861 1.861 0.2 ns
3 Study 1 Inf 4.575 4.575 0.03 *
4 GA_samp 1 Inf 0.008 0.008 0.9 ns
2 folate_Active.B12:Study 1 Inf 0.769 0.769 0.4 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 2.240 2.240 0.1 ns
3 Study 1 Inf 10.426 10.426 0.001 **
4 GA_samp 1 Inf 0.075 0.075 0.8 ns
2 folate_Active.B12:Study 1 Inf 0.861 0.861 0.4 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 8.943 8.943 0.003 **
3 Study 1 Inf 76.835 76.835 <0.0001 ***
4 GA_samp 1 Inf 1.630 1.630 0.2 ns
2 folate_Active.B12:Study 1 Inf 0.099 0.099 0.8 ns

14.9.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 18.89 (11.75, 30.37) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.05
StudySTOP 0.79 (0.68, 0.93) 0.006
GA_samp 1.14 (1.11, 1.18) <0.0001
folate_Active.B12:StudySTOP 1.00 (1.00, 1.00) 0.01
hPL (μg/ml)
Var Effect P
(Intercept) 1.95 (1.52, 2.50) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.52
StudySTOP 1.13 (1.04, 1.21) 0.002
GA_samp 1.33 (1.31, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.07 (0.05, 0.10) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.47
StudySTOP 0.97 (0.88, 1.07) 0.55
GA_samp 1.31 (1.28, 1.33) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 16.48 (8.93, 30.43) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.23
StudySTOP 0.74 (0.61, 0.89) 0.002
GA_samp 1.00 (0.96, 1.04) 0.92
HOMA-IR
Var Effect P
(Intercept) 4.49 (2.33, 8.67) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.19
StudySTOP 0.63 (0.51, 0.77) <0.0001
GA_samp 0.99 (0.95, 1.04) 0.79
Glucose (mmol/L)
Var Effect P
(Intercept) 6.11 (5.35, 6.99) <0.0001
folate_Active.B12 1.00 (1.00, 1.00) 0.003
StudySTOP 0.85 (0.83, 0.88) <0.0001
GA_samp 0.99 (0.98, 1.00) 0.20

14.9.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:Active B12 ratio and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 103.60903 8.502238 94.11762 88.0314 121.9432
STOP 92.11878 7.303106 82.09510 78.6785 107.8550
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 93.00782 2.717590 191.0850 87.79903 98.52562
STOP 104.70916 2.911584 154.5832 99.11265 110.62169
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.427344 0.0799604 354.2074 2.275072 2.589806
STOP 2.353628 0.0710247 276.0152 2.217881 2.497683
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 16.23155 1.0039454 Inf 14.37844 18.32348
STOP 11.98054 0.6055347 Inf 10.85060 13.22814
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.966494 0.2641498 Inf 3.481135 4.519525
STOP 2.493776 0.1339340 Inf 2.244614 2.770595
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.555065 0.0631408 Inf 5.432679 5.680207
STOP 4.736358 0.0530073 Inf 4.633597 4.841399

14.9.4 Comparisons

Effect for every 500 unit increase in Folate:Active B12 ratio.
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE 0.96 (0.93, 1.00) 0.05
PRL (ng/ml) folate_Active.B121123.57 / folate_Active.B12623.57 STOP 1.03 (0.99, 1.07) 0.1
hPL (μg/ml) folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE 1.00 (0.98, 1.01) 0.5
hPL (μg/ml) folate_Active.B121123.57 / folate_Active.B12623.57 STOP 1.00 (0.98, 1.01) 0.5
GH2 (ng/ml) folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE 0.99 (0.97, 1.01) 0.5
GH2 (ng/ml) folate_Active.B121123.57 / folate_Active.B12623.57 STOP 0.99 (0.97, 1.01) 0.5
Insulin (uU/ml) folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE 0.98 (0.94, 1.01) 0.2
Insulin (uU/ml) folate_Active.B121123.57 / folate_Active.B12623.57 STOP 0.98 (0.94, 1.01) 0.2
HOMA-IR folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE 0.97 (0.93, 1.01) 0.2
HOMA-IR folate_Active.B121123.57 / folate_Active.B12623.57 STOP 0.97 (0.93, 1.01) 0.2
Glucose (mmol/L) folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE 0.99 (0.99, 1.00) 0.003
Glucose (mmol/L) folate_Active.B121123.57 / folate_Active.B12623.57 STOP 0.99 (0.99, 1.00) 0.003

14.9.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.9.6 Plots